Liberal education, by all of these value-laden processes, should con- fer a sense of peace, confidence, and assurance on the per- son thus educated and liberate him or her from the adriftness that characterizes so many in an age of anomie. This, too, is a value judgement and a liberation from valuelessness, insecurity, and despair at times. Liberal education should also enable a person to situate himself or herself within a given culture, religion, race, sex, and, hopefully, to appreciate what is valuable in the given situa- tion, even as simple an evaluation as " black is beautiful ". To evaluate is to prefer, to discriminate, to choose, and each of these actions presupposes a sense of values. A liberal educa- tion should also enable a person to judge, which in itself presupposes the ability to evaluate: to prefer this to that, to say this is good and that is bad, or at least this is better than that. Moreover, allied with this release from stupidity non-thinking or poor-thinkingis the freedom to communicate one ' s thoughts, hopefully with clarity, style, and grace, more than the Neanderthal grunt.
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The first fruit of a liberal education is to free a person from ignorance which fundamentally means freedom to think, clearly and logically. What? Excuse me for making a list, but it is important. To be liberal, an education must somehow liberate a person actual- ly to be what every person potentially is: free. rfjp ' - : I- It 1 I : i Do values really count in a liberal education? They have to count if you take the word " liberal " at its face value. i 17 " REN ? THE FICHTINC IRISH ID iii ilii iiiii U. THE DOME 1973 r OT RE 04AI MB for- fc WtlSKZ T5 v T Jf ! ite - ! II r J n. Paulius ORGANIZATIONS EDITOR ' ' ', -.p).
coli closer to that ideal of the perfect host.Text from Pages 1 - 344 of the 1973 volume: “ Michael J. coli expression strains available and move E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. Practical and cutting-edge, Heterologous Gene Expression in E. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. coli expressed proteins, to identify binding partners, and to express membrane proteins. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. Despite the advanced state of protein expression technology improvements are still needed. Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry.
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